Coding

Part:BBa_K1711000:Design

Designed by: Anastasia Nicolov   Group: iGEM15_Washington   (2015-09-17)


AFB2 auxin-binding F-box protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 591
    Illegal XbaI site found at 804
    Illegal XbaI site found at 1242
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1204
    Illegal XhoI site found at 1357
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 591
    Illegal XbaI site found at 804
    Illegal XbaI site found at 1242
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 591
    Illegal XbaI site found at 804
    Illegal XbaI site found at 1242
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 611
    Illegal BsaI site found at 1306
    Illegal SapI.rc site found at 307


Design Notes

The parts sequence had to be checked for illegal restriction sites.


Source

AFB2 was originally found in the genome of the species Arabidopsis thaliana, a small flowering plant.

References